Lactobacillus casei is propagated under essentially anaerobic conditions to provide a starter culture for manufacture of Swiss cheese. The culture produces lactic acid as a by-product of energy metabolism. The system has the following characteristics:

                                                                 

 

A stirred fermenter is operated in fed-batch mode at quasisteady state with a feed flow rate of 4 m³ h^-1 and feed substrate concentration of 80 kg m^-3. After 6 h, the liquid volume is 40 m³. What mass of cells is produced after 6 h fed-batch operation?

                               __________________

TLS Online TPP Program

#Question id: 14281

#Section 5: Bioprocess Engineering and Process Biotechnology

cells are grown in a CSTR a steady state at two dilution rates 0.1 h^-1 and 0.5h^-1 and the steady state concentrations are as follows: 

if the inlet substrate concentration is 20 g/I, the cells follow monod growth kinetics and product formation kinetics is modelled as a both growth associated and non growth associated kinetics given by the equation then answer the following questions, the best estimate of maximum specific growth rate is: 

TLS Online TPP Program

#Question id: 14282

#Section 5: Bioprocess Engineering and Process Biotechnology

The oxygen transfer rate in an aerobic fermentation process does not depend on the

TLS Online TPP Program

#Question id: 14283

#Section 5: Bioprocess Engineering and Process Biotechnology

Aeration ina fermenter is expressed as VVM. What will be the VVM if air is sparged at 2000 L/min with a working volume 10 m^3? 

TLS Online TPP Program

#Question id: 14284

#Section 5: Bioprocess Engineering and Process Biotechnology

The specific productivity (qp) of an enzyme production Is fitted linearly with specific growth rate p of a fungal organism according to the equation The estimated values of constants alpha and beta are 0.0006 and 25 respectively. The enzyme production kinetics is 

TLS Online TPP Program

#Question id: 14285

#Section 5: Bioprocess Engineering and Process Biotechnology

Match the transport process with appropriate time constants given below 

Transport Process	Time Constants
1. Oxygen transfer	p. V/Q
2. Heat transfer	q. 4V(1.5ND3)
3. Flow	r. VpCp/UA
4. Mixing	s. 1/KLa

TLS Online TPP Program

#Question id: 14286

#Section 5: Bioprocess Engineering and Process Biotechnology

 When a liquid of density 1200kg/m3 and viscosity 0.01 kg m^-1s^-1 flows through a 1 cm ID pipe at 0.2m/s, the flow is referred as a