A two-stage chemostat system is used for production of secondary metabolite. The volume of each reactor is 0.5 m³; the flow rate of feed is 50 l h^{- 1}. Mycelial growth occurs in the first reactor; the second reactor is used for product synthesis. The concentration of substrate in the feed is 10 gl^-1. Kinetic and yield parameters for the organism are:

                                                            

Assume that product synthesis is negligible in the first reactor and growth is negligible in the second reactor. Determine cell concentrations entering the second reactor.

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#Question id: 404

#Section 4: Fundamentals of Biological Engineering

When a mixture of 3-phosphoglycerate and 2-phosphoglycerate is incubated at 25 °C with phosphoglycerate mutase until equilibrium is reached, the final mixture contains six times as much 2-phosphoglycerate as 3-phosphoglycerate.  Which one of the following statements is most nearly correct, when applied to the reaction as written?  (R = 8.315 J/mol·K; T = 298 K)  3-Phosphoglycerate -> 2-phosphoglycerate

#Question id: 405

#Section 4: Fundamentals of Biological Engineering

When a mixture of glucose 6-phosphate and fructose 6-phosphate is incubated with the enzyme phosphohexose isomerase (which catalyzes the interconversion of these two compounds) until equilibrium is reached, the final mixture contains twice as much glucose 6-phosphate as fructose 6-phosphate.  Which one of the following statements is best applied to this reaction outlined below? (R = 8.315 J/mol·K; T = 298 K) Glucose 6-phosphate -> fructose 6-phosphate

#Question id: 406

#Section 2: General Biology

Hydrolysis of 1 M glucose 6-phosphate catalyzed by glucose 6-phosphatase is 99% complete at equilibrium (i.e., only 1% of the substrate remains). Which of the following statements is most nearly correct? (R = 8.315 J/mol·K; T = 298 K)

#Question id: 406

#Section 4: Fundamentals of Biological Engineering

Hydrolysis of 1 M glucose 6-phosphate catalyzed by glucose 6-phosphatase is 99% complete at equilibrium (i.e., only 1% of the substrate remains). Which of the following statements is most nearly correct? (R = 8.315 J/mol·K; T = 298 K)

#Question id: 407

#Section 2: General Biology

The reaction A + B -> C has a DG'° of –20 kJ/mol at 25° C.  Starting under standard conditions, one can predict that:

#Question id: 407

#Section 4: Fundamentals of Biological Engineering

The reaction A + B -> C has a DG'° of –20 kJ/mol at 25° C.  Starting under standard conditions, one can predict that: