Ethanol is to be used as a substrate for single-cell protein production in a chemostat. The available equipment can achieve an oxygen transfer rate of 10 g O₂/l of liquid per hour. Assume the kinetics of cell growth on ethanol is of the Monod type, with µm = 0.5 h^–1, K_s = 30 mg/l, YX/S = 0.5 cells/g ethanol, and YO2/S = 2 g O2/g EtOH. We wish to operate the chemostat with an ethanol concentration in the feed of 22 g/L. We also wish to maximize the biomass productivity and minimize the loss of unused ethanol in the effluent. Determine the required dilution rate

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TLS Online TPP Program

#Question id: 19483

#Section 4: Fundamentals of Biological Engineering

Propane (C₃H₈) burns in this reaction:

C₃H₈ + 5O₂ = 4H₂O + 3CO₂

If 200 g of propane is burned, how many g of H2O is produced?

TLS Online TPP Program

#Question id: 19484

#Section 4: Fundamentals of Biological Engineering

Estimate the degree of reduction of Methane, Glucose and Ethanol?

TLS Online TPP Program

#Question id: 19485

#Section 4: Fundamentals of Biological Engineering

The growth of S.cerevisiae on glucose under anaerobic conditions can be described by the following reaction:

C₆H₁₂O₆ + aNH₃ → 0.59 CH1.64N0.16O0.52 (biomass) + 0.43 C3H8O3 + 1.54CO2 + 1.3C2H5OH +0.036H2O

Determine the biomass (MW= 23.74) yield coefficient Yx/s

TLS Online TPP Program

#Question id: 19486

#Section 4: Fundamentals of Biological Engineering

The growth of S.cerevisiae on glucose under anaerobic conditions can be described by the following reaction:

C₆H₁₂O₆ + aNH₃ → 0.59 CH_1.64N_0.16O_0.52 (biomass) + 0.43 C₃H₈O₃ + 1.54CO₂ + 1.3C₂H₅OH + 0.036H₂O 

and determine the product yield coefficient Y etoh/s and determine coefficient a.

TLS Online TPP Program

#Question id: 19487

#Section 4: Fundamentals of Biological Engineering

What is the yield coefficient if the initial substrate concentration is 20 g/l and biomass is 0.2 g/l. The substrate is then consumed and produces 6.0 g/l of biomass?

TLS Online TPP Program

#Question id: 19488

#Section 4: Fundamentals of Biological Engineering

What is the yield coefficient if the initial substrate concentration is 10 g/l and biomass is 0.1 g/l. The substrate is then consumed and produces 5.3 g/l of biomass?