system for manufacture of single-cell protein is Methylophilus methylotrophus. This organism is produced aerobically from methanol with ammonia as nitrogen source. The molecular formula for the biomass is CH _1.680_0.36N_0.22; these cells contain 6% ash. If the actual yield of biomass from methanol is 42% the thermodynamic maximum, what is the oxygen demand?

TLS Online TPP Program

#Question id: 14357

#Section 5: Bioprocess Engineering and Process Biotechnology

Milk is pasteurized if it is heated to 630C for 30 min, but if it is heated to 74°C it only needs 15 s for the same result. Find the activation energy of this sterilization process.  J/mol 

TLS Online TPP Program

#Question id: 14358

#Section 5: Bioprocess Engineering and Process Biotechnology

One liter per minute of liquid containing A and B (CA0 = 0.10 mollliter, CB() = 0.01 mollliter, Cco=0) flow into a mixed reactor of volume V = 1 liter. The materials react in a complex manner for which the stoichiometry is unknown. The outlet stream from the reactor contains A, B, and C (CAf = 0.02 mollliter, CBf = 0.03 mollliter, Cci = 0.04 mollliter), as shown in fig. 

Find the rate of reaction of A, B, and C for the conditions within the reactor  mol/liter.min 

TLS Online TPP Program

#Question id: 14359

#Section 5: Bioprocess Engineering and Process Biotechnology

How many enzyme units are there in 1 katal? 

TLS Online TPP Program

#Question id: 14360

#Section 5: Bioprocess Engineering and Process Biotechnology

1 pg of an enzyme (Mr 160 000) converts 0.192 pmol/min. This enzyme is made up of four subunits. Calculate turnover number 

TLS Online TPP Program

#Question id: 14361

#Section 5: Bioprocess Engineering and Process Biotechnology

1 m of an enzyme (Mr 160 000) converts 0.192 i.imol/min. This enzyme is made up of four  subunits. Calculate catalytic centre activity

TLS Online TPP Program

#Question id: 14362

#Section 5: Bioprocess Engineering and Process Biotechnology

An experiment requires the use of 5 x 107 units of enzyme. The price for the enzyme is quoted for katal amounts. Allowing for a 20% excess for wastage, pipetting, and so forth, what approximate round number of katals of enzyme would you purchase?