Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CH_I.550_0.31N_0.25. The yield ofbiomass from glucose is measured at 0.48 g g^-1; the yield of recombinant protein from glucose is about 20% that for cells. How much ammonia is required per gmol glucose?

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#Question id: 14697

#Section 1: Engineering Mathematics

Two persons each make a single throw with a dice. The probability they get equal value is P1. Four persons each make a single throw and probability of three being equal is P2. Then

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#Question id: 17665

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

BLASTx is used to

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#Question id: 14257

#Section 5: Bioprocess Engineering and Process Biotechnology

The kLa of a small bubble column (2 l) has been measured as 20 h-1 at an airflow of 4 l/m in. If the rate of oxygen uptake by a culture of Catharanthus roseus is 0.2 mmol O2/g dry weight-h and if the critical oxygen concentration must be above 10% of saturation (about 8 mg/l), what is the maximum concentration of cells that can be maintained in the reactor?

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#Question id: 13031

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Use of alkaline solution in southern blotting helps in :

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#Question id: 14788

#Section 1: Engineering Mathematics

The value of  , where [.] is the greatest integer function