Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CHI.5500.31N0.25. The yield of biomass from glucose is measured at 0.48 g g^-1; the yield of recombinant protein from glucose is about 20% that for cells. If the biomass yield remains at 0.48 g g^{- l}, how much change in oxygen requirement for wild-type E. coli unable to synthesise recombinant protein?

TLS Online TPP Program

#Question id: 14353

#Section 5: Bioprocess Engineering and Process Biotechnology

in sparged mammalian cell bioreactors, the cell damage is caused because of

TLS Online TPP Program

#Question id: 14354

#Section 5: Bioprocess Engineering and Process Biotechnology

deceleration phase comes after with phase in growth curve. 

TLS Online TPP Program

#Question id: 14355

#Section 5: Bioprocess Engineering and Process Biotechnology

 Lactic acid fermentation is example of 

TLS Online TPP Program

#Question id: 14356

#Section 5: Bioprocess Engineering and Process Biotechnology

 In Luedeking—Piret equation, if = 0 then product is 

TLS Online TPP Program

#Question id: 14357

#Section 5: Bioprocess Engineering and Process Biotechnology

Milk is pasteurized if it is heated to 630C for 30 min, but if it is heated to 74°C it only needs 15 s for the same result. Find the activation energy of this sterilization process.  J/mol 

TLS Online TPP Program

#Question id: 14358

#Section 5: Bioprocess Engineering and Process Biotechnology

One liter per minute of liquid containing A and B (CA0 = 0.10 mollliter, CB() = 0.01 mollliter, Cco=0) flow into a mixed reactor of volume V = 1 liter. The materials react in a complex manner for which the stoichiometry is unknown. The outlet stream from the reactor contains A, B, and C (CAf = 0.02 mollliter, CBf = 0.03 mollliter, Cci = 0.04 mollliter), as shown in fig. 

Find the rate of reaction of A, B, and C for the conditions within the reactor  mol/liter.min