Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CHI.5500.31N0.25. The yield of biomass from glucose is measured at 0.48 g g^-1; the yield of recombinant protein from glucose is about 20% that for cells. If the biomass yield remains at 0.48 g g^{- l}, how much change in oxygen requirement for wild-type E. coli unable to synthesise recombinant protein?

#Question id: 636

#Section 2: General Biology

DNA polymerase contains a lysine residue that is important for binding to DNA. Mutations were found that converted this lysine to either glutamate, glycine, valine, or arginine. Which mutations would be predicted to be the most and least harmful to the ability of the enzyme to bind DNA?

#Question id: 638

#Section 2: General Biology

Match the following class of enzymes with them

#Question id: 639

#Section 2: General Biology

Match the coenzymes in column I enzymes to class of enzymes in column II

#Question id: 640

#Section 2: General Biology

The data in the table above were collected for an enzyme-catalyzed reaction. The Km for this enzyme is approximately

#Question id: 641

#Section 2: General Biology

Which of the following does NOT describe a mechanism that cells use to regulate enzyme activities?

A. Cells control enzyme activity by phosphorylation and dephosphorylation.

B. Cells control enzyme activity by the binding of small molecules.

C. Cells control the rates of diffusion of substrates to enzymes.

D. Cells control the rates of enzyme degradation.

E. Cells control the rates of enzyme synthesis.

F. Cells control the targeting of enzymes to specific organelles. 

#Question id: 642

#Section 2: General Biology

The graphs P, Q and R show the variation of rate constant (k) with temperature. The reactions represented by P, Q and R, respectively, are