#Question id: 14194
#Section 5: Bioprocess Engineering and Process Biotechnology
Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the culture volume at t = 10 h
#Question id: 14195
#Section 5: Bioprocess Engineering and Process Biotechnology
Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the concentration of glucose at t = 10 h at quasi-steady state
#Question id: 14196
#Section 5: Bioprocess Engineering and Process Biotechnology
A fluidized-bed, immobilized-cell bioreactor is used for the
conversion of glucose to ethanol by Z. mobilis cells immobilized in
k-carrageenan gel beads. The dimensions of the bed are 10 cm (diameter) by 200
cm. Since the reactor is fed from the bottom of the column and because of CO2
gas evolution, substrate and cell concentrations decrease with the height of
the column. The average cell concentration at the bottom of the column is X0
= 45 g/l, and the average cell concentration decreases with the column
height according to the following equation:
where Z is the column height (cm). The specific rate of
substrate consumption is qS = 2 g S/g cells. h. The feed flow rate
and glucose concentration in the feed are 5 l/h and 160 g glucose/l,
respectively.
Determine the substrate (glucose) concentration in the effluent
#Question id: 14197
#Section 5: Bioprocess Engineering and Process Biotechnology
#Question id: 14198
#Section 5: Bioprocess Engineering and Process Biotechnology
which of the following do not come under continuous filtration?
#Question id: 14199
#Section 5: Bioprocess Engineering and Process Biotechnology