A purified hydrolytic enzyme (Mr 150 000) is available with the description: Gives a Vmax of 3 200 units/mg protein under optimal conditions. How many micromoles of substrate can be hydrolysed by 1 mg of protein in 1 minute  

TLS Online TPP Program

#Question id: 13031

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Use of alkaline solution in southern blotting helps in :

TLS Online TPP Program

#Question id: 13032

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In SDS-PAGE, protein sample is first treated with detergent sodium dodecyl sulfate (SDS), in order to

TLS Online TPP Program

#Question id: 13033

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The sample is then fractionated by electrophoresis through a _______. As all the proteins now have an _______ to mass ratio, they are separated on the basis of their _____.

TLS Online TPP Program

#Question id: 13034

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which the following statements is/are true about the stacking gel?

i. The stacking gel solution contains acrylamide.

ii. It is on the top of the separating gel.

iii. It is used to dilute the protein sample before it enters the main separating gel.

iv. It is into this gel that the wells are formed and the proteins loaded.

TLS Online TPP Program

#Question id: 13035

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Each band on the gel represents a different protein (or protein subunit); _____ proteins move through the gel more _____ than_____ proteins and therefore are found nearer the _____ of the gel.

TLS Online TPP Program

#Question id: 13036

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In isoelectric focusing the protein will move until