Practice Questions

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#Question id: 3515

What proportional of F2 round seeded plant would produce all round plant after self fertilization?

#Section 3: Genetics, Cellular and Molecular Biology

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1/3
2/3
1/5

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TLS Online TPP Program

#Question id: 13090

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

What is the Colony 3’s plasmid is;

TLS Online TPP Program

#Question id: 3892

#Section 3: Genetics, Cellular and Molecular Biology

The DNA binding motif for many prokaryotic regulatory proteins, such as the lac repressor, is:

TLS Online TPP Program

#Question id: 3905

#Section 3: Genetics, Cellular and Molecular Biology

Which is not a property of RNA polymerase?

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#Question id: 10791

#Section 6: Plant, Animal and Microbial Biotechnology

Following are certain statements regarding furanocoumarins;

a) furanocoumarins have an attached furan ring

b) These compounds are toxic until they are activated by sunlight in the ultraviolet A (UV-A) region (320–400 nm)

c) Light activated furanocoumarins can insert themselves into the double helix of DNA and bind to the pyrimidine bases, thus blocking transcription and repair and leading eventually to cell death

d) Phototoxic furanocoumarins are especially abundant in members of the Umbelliferae family, including celery, parsnip, and parsley.

Which of the following combination from the above statements is correct?

TLS Online TPP Program

#Question id: 5925

#General Aptitude

On what dates of April, 2001 did Wednesday fall ?