The single-stranded bubble created in DNA replication is coated with _____________ to protect it from breakage and to prevent the DNA renaturing.

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#Question id: 13087

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

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#Question id: 183

#Section 2: General Biology

At pH 7.0, converting a glutamic acid to gamma-carboxyglutamate, will have what effect on the overall charge of the protein containing it?

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#Question id: 15684

#Section 1: Engineering Mathematics

If  then (x , y)

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#Question id: 12926

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

PCR is undertaken with a fluorescence reporter / quencher pair called______

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#Question id: 635

#Section 2: General Biology

The enymatic rate constant (kcat/Km) of orotidine 5ʹ-phosphate decarboxylase is 6 x 10⁷ M^-1s^-1 and the nonenzymatic rate constant (kn) is 3 x 10^-16 s^-1.  What is the value of the enzymeʹs catalytic proficiency?