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TLS Online TPP Program

#Question id: 3719

The processivity of E. coli DNA polymerase III holoenzyme accounts for all of the following except

#Section 3: Genetics, Cellular and Molecular Biology

  1. the relatively small number of enzymes needed to replicate the entire chromosome.

  2. the directionality of polymerization.

  3. the rapid rate of polymerization.

  4. the ability of a single holoenzyme to add many nucleotides to a growing DNA chain.

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TLS Online TPP Program

#Question id: 12912

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the enzyme which separates the double-helical DNA into single strands.
TLS Online TPP Program

#Question id: 12913

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

________  involves the size analysis of restriction fragments produced by several restriction enzymes individually and in combination. 
TLS Online TPP Program

#Question id: 12914

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 In Electrophoresis of DNA the gel is first soaked in the alkali then it is transferred to the membrane so that the ________
TLS Online TPP Program

#Question id: 12915

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following statement is not correct about PCR.
TLS Online TPP Program

#Question id: 12916

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which statement is correct for Zoo blotting?
TLS Online TPP Program

#Question id: 12917

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Arrange the steps of stages in PCR in correct order.
1. The region to be specifically amplified is made accessible within the complex DNA.
2. The double stranded DNA is denatured by heating the reaction mixture to above 90 degree Celsius.
3. A thermal cycler is used to incrementally decrease the annealing temperature.
4. Reaction mixture is then cooled to a temperature between 40 and 60 degree celsius.