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TLS Online TPP Program

#Question id: 145

The pKaʹs of isoleucineʹs α-Carboxyl group and α-Amino group are 2.3 and 9.8, respectively. Calculate the isoelectric point.

#Section 2: General Biology

  1. 2.3

  2.  6.0

  3. 9.8

  4. The isoelectric point cannot be calculated without the pKa value for the side chain.

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TLS Online TPP Program

#Question id: 3318

#Section 3: Genetics, Cellular and Molecular Biology

When members of a metal- torerant and metal sensitive species that initially occupied the same habitat within the same range diverge into two species due to starting to show a change in their flowering season. This process known as
TLS Online TPP Program

#Question id: 13091

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
                              
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
 
Which colony’s plasmid do you actually want to use for your studies?
TLS Online TPP Program

#Question id: 15551

#Section 1: Engineering Mathematics

Compute the root of function f(x) = x2 - 3 after two iterations using bisection method in the interval [0, 2] 
TLS Online TPP Program

#Question id: 14230

#Section 5: Bioprocess Engineering and Process Biotechnology

In current service, 20 kg s^-1 cooling water at 12⁰C must be circulated through a coil inside a fermenter to maintain the temperature at 37⁰C The coil is 150 m long with pipe diameter 12 cm; the exit water temperature is 28⁰C After the inner and outer surfaces of the coil are cleaned it is found that only 13 kg s^-1 cooling water is required to control the fermentation temperature. Calculate the overall heat-transfer coefficient before cleaning. _____________
TLS Online TPP Program

#Question id: 12998

#Section 2: General Biology

Match with appropriate bonding pattern