Titration of valine by a strong base, for example NaOH, reveals two pK’s. The titration reaction occurring at pK2 (pK2 = 9.62) is:

TLS Online TPP Program

#Question id: 12911

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:

A. DNA polymerases	i. Alkaline phosphatase	a. RNA-dependent DNA polymerase, used in cDNA synthesis
B. Reverse transcriptase	ii. T4 DNA ligase	b. Thermostable DNA polymerase used in PCR
C. Ligases	iii. AMV-RT	c. Removes 5'-phosphates from DNA and RNA
D. Phosphatases	iv. Taq DNA pol	d. Links 5'-phosphate and 3'-hydroxyl ends via phosphodiester bond

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#Question id: 12912

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the enzyme which separates the double-helical DNA into single strands.

TLS Online TPP Program

#Question id: 12913

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

________  involves the size analysis of restriction fragments produced by several restriction enzymes individually and in combination. 

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#Question id: 12914

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 In Electrophoresis of DNA the gel is first soaked in the alkali then it is transferred to the membrane so that the ________

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#Question id: 12915

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following statement is not correct about PCR.

TLS Online TPP Program

#Question id: 12916

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which statement is correct for Zoo blotting?