Suppose the major solutes in intact lysosomes are KCl (~0.1 M) and NaCl (~0.03 M). When isolating lysosomes, what concentration of sucrose is required in the extracting solution at room temperature (25 °C) to prevent swelling and lysis?

#Question id: 18085

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 19462

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 15939

#Section 1: Engineering Mathematics

 

#Question id: 14274

#Section 5: Bioprocess Engineering and Process Biotechnology

Nicotiana tabacum cells are cultured to high density for production of polysaccharide gum. The reactor used is a stirred tank, containing initially 100 litres medium. The maximum specific growth rate of the culture is 0.18 d^-1 and the yield of biomass from substrate is 0.5 g g^-l. The concentration of growth-limiting substrate in the medium is 3% (w/v). The reactor is inoculated with 1.5 g l^{- 1} cells and operated in batch until the substrate is virtually exhausted; medium flow is then started at a rate of 4 l d^-1. Fed-batch operation occurs under quasi-steady-state conditions. Estimate final biomass concentration?

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#Question id: 1841

#Section 2: General Biology

The circulation of a 2-month-old breast-fed baby will contain maternal: