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TLS Online TPP Program

#Question id: 3855

In eukaryotes, which of the following modifications is made to a primary transcript prior to its transport out of the nucleus?

#Section 3: Genetics, Cellular and Molecular Biology

  1. poly(A) tails added to the 3' end 

  2. 7-methyl guanosine caps added to the 5' end

  3. exon sequences, when present, are spliced out 

  4. all of these

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TLS Online TPP Program

#Question id: 14324

#Section 5: Bioprocess Engineering and Process Biotechnology

Production of recombinant protein by a geneticallyengineered strain of Escherichia coli is proportional to cell growth. Ammonia is used as nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula CHI.5500.31N0.25. The yield of biomass from glucose is measured at 0.48 g g-1; the yield of recombinant protein from glucose is about 20% that for cells. If the biomass yield remains at 0.48 g g- l, how much change in oxygen requirement for wild-type E. coli unable to synthesise recombinant protein?
TLS Online TPP Program

#Question id: 13082

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Statement: AFLP shares some features of both RFLP and RAPD analyses.
Explanations: I. It uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.
II. AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.
TLS Online TPP Program

#Question id: 14240

#Section 5: Bioprocess Engineering and Process Biotechnology

A 200-1itre stirred fermenter contains a batch culture of Bacillus subtilis bacteria at 28⁰C Air at 20⁰C is pumped into the vessel at a rate of 1 vvm; (vvm stands for volume of gas per volume of liquid per minute). The average pressure in the fermenter is 1 atm. The volumetric flow rate of off-gas from the fermenter is measured as 189 l min-1. The exit gas stream is analysed for oxygen and is found to contain 20.1% 02. The dissolved-oxygen concentration in the broth is measured using an oxygen electrode as 52% air saturation. The solubility of oxygen in the fermentation broth at 28⁰C and 1 atm air pressure is 7.8 x 10-3 kg m-3. Determine the value of kLa for the system ____________
TLS Online TPP Program

#Question id: 5150

#General Aptitude

If the number 481* 673 is completely divisible by 9, then the smallest whole number in place of * will be : 
TLS Online TPP Program

#Question id: 422

#Section 4: Fundamentals of Biological Engineering

The flow of material through a reaction pathway usually depends on ________.