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TLS Online TPP Program

#Question id: 3863

Introns in nuclear pre-mRNAs are excised by:

#Section 3: Genetics, Cellular and Molecular Biology

  1. spliceosomes

  2. splicing nucleases

  3. ligases

  4. autocatalytic reactions

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TLS Online TPP Program

#Question id: 12733

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

It provides a key marker of potential disease development and reaction to individual treatment.
TLS Online TPP Program

#Question id: 1633

#Section 2: General Biology

Immunological memory can be transferred experimentally by:
TLS Online TPP Program

#Question id: 14314

#Section 5: Bioprocess Engineering and Process Biotechnology

An industrial waste-water stream is fed to a stirred-tank reactor continuously and the cells are recycled back to the reactor from the bottom of the sedimentation tank placed after the reactor. The following are given for the system: F = 100 l/h; S0 = 5000 mg/l; mm = 0.25 h^-1 ; Ks = 200 mg/l; α (recycle ratio) = 0.6; C (cell concentration factor) = 2; Y M X/S = 0.4. The effluent concentration is desired to be 100 mg/l.  The effluent concentration is desired to be 100 mg/l. If the residence time is 2 h in the sedimentation tank, determine the volume of the sedimentation tank and cell concentration in the effluent of the sedimentation tank.
TLS Online TPP Program

#Question id: 406

#Section 4: Fundamentals of Biological Engineering

Hydrolysis of 1 M glucose 6-phosphate catalyzed by glucose 6-phosphatase is 99% complete at equilibrium (i.e., only 1% of the substrate remains). Which of the following statements is most nearly correct? (R = 8.315 J/mol·K; T = 298 K)
TLS Online TPP Program

#Question id: 13090

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
                              
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
 
What is the Colony 3’s plasmid is;