TLS Online TPP Program

#Question id: 3868


The sigma factor of E. coli RNA polymerase: 

#Section 3: Genetics, Cellular and Molecular Biology
  1. associates with the promoter before binding core enzyme.

  2. combines with the core enzyme to confer specific binding to a promoter.

  3. is inseparable from the core enzyme.

  4. is required for termination of an RNA chain.

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TLS Online TPP Program

#Question id: 2125

#Section 2: General Biology

The shortest alpha helix segment in a protein that will span a membrane bilayer has about _____ amino acid residues.

TLS Online TPP Program

#Question id: 15494

#Section 4: Fundamentals of Biological Engineering

Assuming that cells convert two-thirds (w/w) of the substrate to biomass, the stoichiometric coefficient (1 only) for glucose utilisation for the following reaction is

TLS Online TPP Program

#Question id: 3368

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The origin of a new plant species by hybridization, coupled with accidents during nuclear division, is an example of

TLS Online TPP Program

#Question id: 14254

#Section 5: Bioprocess Engineering and Process Biotechnology

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  Determine the height of the packed bed for 95% glucose conversion. Bed diameter is D0 = 0.2 m. Neglect the growth of the hybridomas and assume first order kinetics. 

TLS Online TPP Program

#Question id: 15759

#Section 6: Plant, Animal and Microbial Biotechnology

Somatic hybrids exhibit the full somatic complement of one parental species, while all or nearly all chromosomes of the other parental species are lost during the preceding mitotic divisions; such hybrids arc referred to as