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TLS Online TPP Program

#Question id: 563

Both water and glucose share an —OH that can serve as a substrate for a reaction with the terminal phosphate of ATP catalyzed by hexokinase.  Glucose, however, is about a million times more reactive as a substrate than water.  The best explanation is that:

#Section 2: General Biology

  1. glucose has more —OH groups per molecule than does water.

  2. the larger glucose binds better to the enzyme; it induces a conformational change in hexokinase that brings active-site amino acids into position for catalysis.

  3. the —OH group of water is attached to an inhibitory H atom, while the glucose —OH group is attached to C.

  4. water and the second substrate, ATP, compete for the active site resulting in a competitive inhibition of the enzyme.

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TLS Online TPP Program

#Question id: 13066

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following is a codominant marker?
TLS Online TPP Program

#Question id: 13067

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The first immunoassay formats described were methods based on the ____ reaction, which is characterised either by gel formation in a liquid phase or as an opaque band in an agar plate assay.
TLS Online TPP Program

#Question id: 13068

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Coating antibody; which actively traps antigen
TLS Online TPP Program

#Question id: 13069

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

A negative agglutination test may NOT indicate the
TLS Online TPP Program

#Question id: 13070

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Antibodies raised against an antigen from one organism will react to a greater or lesser degree with similar antigens from a related organism, depending on how many surface epitopes they share. This has given rise to a systematic method of identification of microorganisms known as______?
TLS Online TPP Program

#Question id: 13071

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Modifications of the agglutination reaction involve the use of ________particles, which allow the reaction to be observed more easily in the liquid phase