Protein A has four identical subunits, each of which binds one molecule of ligand Y. The binding of one molecule of Y to one of the subunits induces a conformational change in neighboring subunits that enhances the binding of additional units of Y. This is an example of________.

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#Question id: 13057

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 3895

#Section 3: Genetics, Cellular and Molecular Biology

Which one of the following types of eukaryotic regulatory proteins interact with enhancers?

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#Question id: 1181

#Section 3: Genetics, Cellular and Molecular Biology

Serum response factor (SRF) is phosphorylated by:

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#Question id: 14281

#Section 5: Bioprocess Engineering and Process Biotechnology

cells are grown in a CSTR a steady state at two dilution rates 0.1 h^-1 and 0.5h^-1 and the steady state concentrations are as follows: 

if the inlet substrate concentration is 20 g/I, the cells follow monod growth kinetics and product formation kinetics is modelled as a both growth associated and non growth associated kinetics given by the equation then answer the following questions, the best estimate of maximum specific growth rate is: 

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#Question id: 15598

#Section 1: Engineering Mathematics

The function f(x) = e^x - 1 is to be solved using Newton-Raphson method. If the initial value of xo is taken as 1.0, then the absolute error observed at 2^nd iteration is .