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TLS Online TPP Program

#Question id: 10132

Which of the following statements would be correct for photophosphorylation ?

#Section 2: General Biology
  1. Water is oxidized and protons are released in the lumen by PSII and reduces NADP+ to NADPH in the stroma both are spontaneous processes becoz their reduction potential is positive
  2. The sequential absorption of four photons (excitons) —known as S states, each absorption causing the loss of one electron from the Mn4Ca cluster, produces an oxidizing agent that can remove four electrons from two molecules of water, producing O2, the electrons lost from the Mn4Ca cluster pass one at a time to an oxidized Tyr residue in a PSII protein, then to P680+
  3. An alternative path of electron is cyclic electron transfer, in which elctron move from ferredoxin back to the cytochrome b6 f complex, the cyclic pathway produces more ATP and Water produces but less NADPH produces than the noncyclic
  4. Transfer of the two electrons to PQB reduces it to PQB 2–, and the reduced PQB 2– takes two protons from the stroma side of the medium, yielding a fully reduced plastohydroquinone (PQH2), the PQH2 then still remain bounded from the reaction center complex
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TLS Online TPP Program

#Question id: 14163

#Section 5: Bioprocess Engineering and Process Biotechnology

A stream of growth medium containing 60% glucose and 40% growth factor A flowing at 500 kg per hour was divided into two feed streams for different cell cultures. One fourth of the growth medium was sent to fermenter 1 and the rest was sent to fermenter 2. What is the flow rates of glucose and growth factor A in the feed streams for the two fermenters
TLS Online TPP Program

#Question id: 14164

#Section 4: Fundamentals of Biological Engineering

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                                  

  Assuming 100% conversion, what is the yield of cells from hexadecane in g g-l?   
TLS Online TPP Program

#Question id: 14164

#Section 5: Bioprocess Engineering and Process Biotechnology

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                                  

  Assuming 100% conversion, what is the yield of cells from hexadecane in g g-l?   
TLS Online TPP Program

#Question id: 14165

#Section 4: Fundamentals of Biological Engineering

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                                    

Assuming 100% conversion, what is the yield of cells from oxygen in g g-l?
TLS Online TPP Program

#Question id: 14165

#Section 5: Bioprocess Engineering and Process Biotechnology

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                                    

Assuming 100% conversion, what is the yield of cells from oxygen in g g-l?
TLS Online TPP Program

#Question id: 14166

#Section 4: Fundamentals of Biological Engineering

Stoichiometric equations are used to represent growth of microorganisms provided a ' molecular formula' for the cells is available. The molecular formula for biomass is obtained by measuring the amounts of C, N, H, O and other elements in cells. For a particular bacterial strain, the molecular formula was determined to be C4.4H7.30 1.2N0.86 . These bacteria are grown under aerobic conditions with hexadecane (C16H34) as substrate. The reaction equation describing growth is:

                             

You have been put in charge of a small fermenter for growing the bacteria and aim to produce 2.5 kg of cells for inoculation of a pilot-scale reactor. What minimum amount of hexadecane substrate must be contained in your culture medium?