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TLS Online TPP Program

#Question id: 1963

Inflammatory responses may include which of the following?

#Section 2: General Biology

  1. clotting proteins migrating away from the site of infection

  2. increased activity of phagocytes in an inflamed area

  3. reduced permeability of blood vessels to conserve plasma

  4. release of substances to decrease the blood supply to an inflamed area

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TLS Online TPP Program

#Question id: 13060

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
In blue white screening insertional activation of Beta-D-galactosidase will give rise to
TLS Online TPP Program

#Question id: 15736

#Section 6: Plant, Animal and Microbial Biotechnology

In shoot regeneration which attribute may have an inhibitory effect on it
TLS Online TPP Program

#Question id: 5844

#Section 3: Genetics, Cellular and Molecular Biology

In homologous genetic recombination, RecA protein is involved in:
TLS Online TPP Program

#Question id: 3655

#Section 3: Genetics, Cellular and Molecular Biology

The mismatched base is removed from the daughter strand of DNA by:
TLS Online TPP Program

#Question id: 14274

#Section 5: Bioprocess Engineering and Process Biotechnology

Nicotiana tabacum cells are cultured to high density for production of polysaccharide gum. The reactor used is a stirred tank, containing initially 100 litres medium. The maximum specific growth rate of the culture is 0.18 d-1 and the yield of biomass from substrate is 0.5 g g-l. The concentration of growth-limiting substrate in the medium is 3% (w/v). The reactor is inoculated with 1.5 g l- 1 cells and operated in batch until the substrate is virtually exhausted; medium flow is then started at a rate of 4 l d-1. Fed-batch operation occurs under quasi-steady-state conditions. Estimate final biomass concentration?

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