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TLS Online TPP Program

#Question id: 1998

Jenner successfully used cowpox virus as a vaccine against the virus that causes smallpox. Why was he successful even though he used viruses of different kinds?

#Section 2: General Biology

  1. The immune system responds nonspecifically to antigens.

  2. The cowpox virus made antibodies in response to the presence of smallpox.

  3. Cowpox and smallpox are antibodies with similar immunizing properties.

  4. There are some antigenic determinants common to both pox viruses.

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TLS Online TPP Program

#Question id: 14358

#Section 5: Bioprocess Engineering and Process Biotechnology

One liter per minute of liquid containing A and B (CA0 = 0.10 mollliter, CB() = 0.01 mollliter, Cco=0) flow into a mixed reactor of volume V = 1 liter. The materials react in a complex manner for which the stoichiometry is unknown. The outlet stream from the reactor contains A, B, and C (CAf = 0.02 mollliter, CBf = 0.03 mollliter, Cci = 0.04 mollliter), as shown in fig. 


Find the rate of reaction of A, B, and C for the conditions within the reactor  mol/liter.min 
TLS Online TPP Program

#Question id: 14359

#Section 5: Bioprocess Engineering and Process Biotechnology

How many enzyme units are there in 1 katal? 
TLS Online TPP Program

#Question id: 14360

#Section 5: Bioprocess Engineering and Process Biotechnology

1 pg of an enzyme (Mr 160 000) converts 0.192 pmol/min. This enzyme is made up of four subunits. Calculate turnover number 
TLS Online TPP Program

#Question id: 14361

#Section 5: Bioprocess Engineering and Process Biotechnology

1 m of an enzyme (Mr 160 000) converts 0.192 i.imol/min. This enzyme is made up of four  subunits. Calculate catalytic centre activity
TLS Online TPP Program

#Question id: 14362

#Section 5: Bioprocess Engineering and Process Biotechnology

An experiment requires the use of 5 x 107 units of enzyme. The price for the enzyme is quoted for katal amounts. Allowing for a 20% excess for wastage, pipetting, and so forth, what approximate round number of katals of enzyme would you purchase? 
TLS Online TPP Program

#Question id: 14363

#Section 5: Bioprocess Engineering and Process Biotechnology

Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate the specific activities in units/g at each of the two steps