What accounts for antibody switching (i.e., the switch of one B cell from producing one class of antibody to another antibody class that is responsive to the same antigen)?

#Question id: 13073

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13074

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13075

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13076

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 13077

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13078

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology