Hardy-Weinberg equilibrium, the frequencies of the two alleles A1 and A2 is 0.4 and 0.6. When you consider A2 is dominant allele of genetic disorder. The frequency of defected individuals, in population size 1000 is

#Question id: 14304

#Section 5: Bioprocess Engineering and Process Biotechnology

Pseudomonas sp. has a mass doubling time of 2.4 h when grown on acetate. The saturation constant using this substrate is 1.3 g/l (which is unusually high), and cell yield on acetate is 0.46 g cell/g acetate. If we operate a chemostat on a feed stream containing 38 g/l acetate, find the Cell productivity at 0.8 Dmax?

_____________

#Question id: 14305

#Section 5: Bioprocess Engineering and Process Biotechnology

Ethanol is to be used as a substrate for single-cell protein production in a chemostat. The available equipment can achieve an oxygen transfer rate of 10 g O₂/l of liquid per hour. Assume the kinetics of cell growth on ethanol is of the Monod type, with µm = 0.5 h^–1, K_s = 30 mg/l, YX/S = 0.5 cells/g ethanol, and YO2/S = 2 g O2/g EtOH. We wish to operate the chemostat with an ethanol concentration in the feed of 22 g/L. We also wish to maximize the biomass productivity and minimize the loss of unused ethanol in the effluent. Determine the required dilution rate

#Question id: 14306

#Section 5: Bioprocess Engineering and Process Biotechnology

Pseudomonas putida with mm = 0.5 h^-1 is cultivated in a continuous culture under aerobic conditions where D = 0.28 h^-1 . The carbon and energy source in the feed is lactose with aconcentration of S₀ = 2 g/l. The effluent lactose concentration is desired to be S = 0.1 g/l. If the growth rate is limited by oxygen transfer, by using the following information:

Determine the steady-state biomass concentration (X)

                _________________

#Question id: 14307

#Section 5: Bioprocess Engineering and Process Biotechnology

Pseudomonas putida with mm = 0.5 h^-1 is cultivated in a continuous culture under aerobic conditions where D = 0.28 h^-1 . The carbon and energy source in the feed is lactose with aconcentration of S₀ = 2 g/l. The effluent lactose concentration is desired to be S = 0.1 g/l. If the growth rate is limited by oxygen transfer, by using the following information

Determine specific rate of oxygen consumption (q_O2 ).

#Question id: 14308

#Section 5: Bioprocess Engineering and Process Biotechnology

Pseudomonas putida with µm = 0.5 h^-1 is cultivated in a continuous culture under aerobic conditions where D = 0.28 h^-1 . The carbon and energy source in the feed is lactose with aconcentration of S₀ = 2 g/l. The effluent lactose concentration is desired to be S = 0.1 g/l. If the growth rate is limited by oxygen transfer, by using the following information:

What should be the oxygen-transfer coefficient (k_La) in order to overcome oxygentransfer limitation (i.e., CL = 2 mg/l)? ___________________

#Question id: 14309

#Section 5: Bioprocess Engineering and Process Biotechnology

Glucose is converted to ethanol by immobilized yeast cells entrapped in gel beads. The specific rate of ethanol production is: q_P = 0.2 g ethanol/g-cell-h. The effectiveness factor for an average bead is 0.8. Each bead contains 50 g/L of cells. The voids volume in the column is 40%. Assume growth is negligible (all glucose is converted into ethanol). The feed flow rate is F = 400 l/h and glucose concentration in the feed is S_0i = 150 g glucose/l. The diameter of the column is 1 m and the yield coefficient is about 0.49 g ethanol/g glucose. The column height is 4 m. What is the glucose conversion at the exit of the column?