With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 

You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.

 

 Why is it that having more repeats leads to a band that is higher in the gel?

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#Question id: 598

#Section 2: General Biology

Which might be a method used for distinguishing among several mechanistic possibilities in a multi-substrate reaction?

TLS Online TPP Program

#Question id: 10776

#Section 6: Plant, Animal and Microbial Biotechnology

pyrethroids that occur in the leaves and flowers of Chrysanthemum species show very striking insecticidal activity. Pyrethroids are________

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#Question id: 1930

#Section 2: General Biology

Which of the following molecules are NOT used by tumor associated macrophages to generate an anti-inflammatory environment within a tumor?

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#Question id: 13052

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

TLS Online TPP Program

#Question id: 13064

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?