To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 12737

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 Name the technique which is used to detect abnormalities at an early stage.

TLS Online TPP Program

#Question id: 12738

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match  the following:

1. Growth Factors                                                    i. GTPase

2. Protein kinases                                                    ii. PDGF

3. G- Proteins                                                           iii. DNA binding protein

4. Nucleus located transcription factors      iv.Serine Kinases

TLS Online TPP Program

#Question id: 12739

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Method used for gene editing and for the development in Gene modifications is known as_______.

TLS Online TPP Program

#Question id: 12809

#General Aptitude

The breadth of a rectangular hall is three-fourth of its length. If the area of the floor is 768 sq. m. then the difference between the length and breadth of the hall is:

TLS Online TPP Program

#Question id: 12819

#Section 2: General Biology

The length and breadth of a rectangle are increased by 20% and 25% respectively. The increase in the area of the resulting rectangle will be :

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#Question id: 12902

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

An extension of microarray technology may also be used to analyze tissue sections,this process is known as_____