To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

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#Question id: 18218

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following is an incorrect statement about the terminologies related to protein sorting?

TLS Online TPP Program

#Question id: 3727

#Section 3: Genetics, Cellular and Molecular Biology

Fragments on the lagging strand are joined by the enzyme ________.

TLS Online TPP Program

#Question id: 2097

#Section 3: Genetics, Cellular and Molecular Biology

Free and unesterified fatty acids bind to one of a number of chaperone proteins in order to be transported in the aqueous environment of the cytosol. Which of the following facilitates this intracellular transport?

TLS Online TPP Program

#Question id: 3748

#Section 3: Genetics, Cellular and Molecular Biology

How are the BRCA proteins and their genes implicated in breast cancer?

TLS Online TPP Program

#Question id: 3757

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following characteristics of eukaryotic telomeres cause them to replicate differently than the rest of the chromosome?