To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

#Question id: 4907

#Section 3: Genetics, Cellular and Molecular Biology

Two corn plants are studied. One is resistant (R) and the other is susceptible (S) to a certain pathogenic fungus. The following crosses are made, with the results shown

R male X S female = all S progeny

R female X S male = all R progeny

Which of the following are correct about inheritance?

#Question id: 4908

#Section 3: Genetics, Cellular and Molecular Biology

Shell coiling of the snail is maternal effect phenotype control by S gene, where S Dextral is dominant over s sinistral. If snail sinistral coiled shell, which of the following statement is true

#Question id: 4909

#Section 3: Genetics, Cellular and Molecular Biology

A pattern of transmission where all offspring have the same phenotype as their mother is consistent with which type of non-Mendelian inheritance?

#Question id: 4910

#Section 3: Genetics, Cellular and Molecular Biology

For a trait known to be caused by a mitochondrial gene variant, there may be an occasional female that can transmit different phenotypes to her progeny on an apparently random basis. What situation might you suspect?

#Question id: 4911

#Section 3: Genetics, Cellular and Molecular Biology

Neutral petite mutations:

#Question id: 4912

#Section 3: Genetics, Cellular and Molecular Biology

Correns described that the inheritance of variegated color on the leaves of certain plants was determined by the maternal parent only. What phenomenon does this describe?