To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

#Question id: 4738

#Section 3: Genetics, Cellular and Molecular Biology

Red kernel color in wheat results from the presence of at least one dominant allele of each of two independently segregating genes . Kernels on rr bb plants are white, and the genotypes R— bb and rr B— result in brown kernel color. Suppose that plants of a variety that is true breeding for red kernels are crossed with plants true breeding for white kernels produced F1 were allowed to selfing .what are F2 phenotype ratio ?

#Question id: 4740

#Section 3: Genetics, Cellular and Molecular Biology

Following metabolic pathway for synthesis of anthocyanin product ? Which of the following cross produced all purple phenotype in their progeny ?

#Question id: 4741

#Section 3: Genetics, Cellular and Molecular Biology

The F2 progeny from a particular cross exhibit a modified dihybrid ratio of 9 : 7 (instead of 9 : 3 : 3 : 1). What phenotypic ratio would be expected from a testcross of the F1?

#Question id: 4745

#Section 3: Genetics, Cellular and Molecular Biology

In some goats, the presence of horns is produced by an autosomal gene that is dominant in males and recessive in females. A horned female is crossed with a hornless male. The F1 offspring are intercrossed to produce the F2. What proportion of the F2 offspring will have horns?

#Question id: 4746

#Section 3: Genetics, Cellular and Molecular Biology

Two independent pure line varieties having deep red and brown phenotype in their seed coat were cross, the all f1 progeny is deep red. The F2 progeny obtain from selfing show deep red and brown in ratio of 15:1. Which of the following observation is correct?

#Question id: 4747

#Section 3: Genetics, Cellular and Molecular Biology

How does incomplete dominance differ from incomplete penetrance?