To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

#Question id: 3750

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following facts did Hershey and Chase make use of in trying to determine whether DNA or protein is the genetic material?

#Question id: 3752

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following investigators was (were) responsible for the discovery that in DNA from any species, the amount of adenine equals the amount of thymine, and the amount of guanine equals the amount of cytosine?

#Question id: 3753

#Section 3: Genetics, Cellular and Molecular Biology

Cytosine makes up 42% of the nucleotides in a sample of DNA from an organism. Approximately what percentage of the nucleotides in this sample will be thymine?

#Question id: 3754

#Section 3: Genetics, Cellular and Molecular Biology

Thymine makes up 28% of the nucleotides in a sample of DNA from an organism. Approximately what percentage of the nucleotides in this sample will be guanine?

#Question id: 3755

#Section 3: Genetics, Cellular and Molecular Biology

In E. coli, there is a mutation in a gene called dnaB that alters the helicase that normally acts at the origin of replication. Which of the following events would you expect to occur as a result of this mutation?

#Question id: 3756

#Section 3: Genetics, Cellular and Molecular Biology

 In E. coli, which enzyme catalyzes the elongation of a new DNA strand in the 5' → 3' direction?