To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

#Question id: 27326

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of a microorganism on glucose is described by the Monod model (µmax = 0.7 h¹ and Ks = 0.1 g L¹). If the initial biomass concentration in the batch fermenter is 0.1 g L¹, the minimum time required to triple the biomass concentration would be

#Question id: 27327

#Section 5: Bioprocess Engineering and Process Biotechnology

#Question id: 27328

#Section 5: Bioprocess Engineering and Process Biotechnology

The unit of maintenance coefficient is

#Question id: 27329

#Section 5: Bioprocess Engineering and Process Biotechnology

Culture comprising a B. subtlis strain by inoculating 1g of cells into 1 liter bioreactor. Fermentor containing 10g/l of glucose. The maximum specific growth rate of the culture is 1.0 /h. The biomass yield from glucose is 0.5 g/g. If the residual glucose concentration left out is 1g/l, the final cell concentration in g/l achieved would be around

#Question id: 27330

#Section 5: Bioprocess Engineering and Process Biotechnology

The ethanol yield per unit mass of substrate consumed (YP/S) in an anaerobic fermentation process can be improved by

#Question id: 27331

#Section 3: Genetics, Cellular and Molecular Biology

RNA molecules that act as enzymes are called as