To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which of the following restriction enzyme is the best choice for you use to design a way to get the insert into the vector?

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#Question id: 583

#Section 2: General Biology

When there are two substrates in a reaction, the reaction is said to be second order if ________.

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#Question id: 14729

#Section 1: Engineering Mathematics

The figure below shows the network connecting cities A, B, C, D, E and F. The arrows indicate permissible directions of travel. If Deepak from city A wants to visit city F, what is the probability he will pass through city C

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#Question id: 19554

#Section 4: Fundamentals of Biological Engineering

What is the unit of specific growth rate?

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#Question id: 14500

#Section 1: Engineering Mathematics

A fair die is tossed 180 times. The expected number of sixes is

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#Question id: 1888

#Section 2: General Biology

In thyroid autoimmunity, an antibody causing type V hypersensitivity may be present and is directed against: