TLS Online TPP Program

#Question id: 13084


Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. II is true, but not correct explanation of given statement.
  2. I and II both are true but not correct explanations of given statement.
  3. I is true and correct explanation of given statement.
  4. I and II both are true and correct explanations of given statement.
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TLS Online TPP Program

#Question id: 15491

#Section 4: Fundamentals of Biological Engineering

The respiratory coefficient for the reaction    is defined as
   

TLS Online TPP Program

#Question id: 15492

#Section 4: Fundamentals of Biological Engineering

Assume that experimental measurements for a certain organism have shown that cells can convert two-thirds (wt/wt) of the substrate carbon to biomass carbon. Calculate the stoichiometric coefficients for the following biological reactions:
 Hexadecane: C16H34 + aO2 + bNH3 c(C4.4H7.3N0.86O1.2) + dH2O + eCO2

TLS Online TPP Program

#Question id: 15493

#Section 4: Fundamentals of Biological Engineering

Assume that experimental measurements for a certain organism have shown that cells can convert two-thirds (wt/wt) of the substrate carbon to biomass carbon. Calculate the stoichiometric coefficients for the following biological reactions:

Glucose: C6H12O6 + aO2 + bNH3 c(C4.4H7.3N0.86O1.2) + dH2O + eCO2

TLS Online TPP Program

#Question id: 15494

#Section 4: Fundamentals of Biological Engineering

Assuming that cells convert two-thirds (w/w) of the substrate to biomass, the stoichiometric coefficient (1 only) for glucose utilisation for the following reaction is

TLS Online TPP Program

#Question id: 15495

#Section 4: Fundamentals of Biological Engineering

Calculate the degree of reduction for following substrates:
A) Methane
B) Glucose
C) Ethanol

TLS Online TPP Program

#Question id: 15496

#Section 4: Fundamentals of Biological Engineering

S. cerevisiae produces ethanol at a yield of 0.5 g/g glucose. The strain ferments 20g/l glucose in 24 hours. Calculate productivity of ethanol in this fermentation