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TLS Online TPP Program

#Question id: 13084

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. II is true, but not correct explanation of given statement.
  2. I and II both are true but not correct explanations of given statement.
  3. I is true and correct explanation of given statement.
  4. I and II both are true and correct explanations of given statement.
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TLS Online TPP Program

#Question id: 14132

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The most widely used program for multiple sequence alignment is
TLS Online TPP Program

#Question id: 14133

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The study of evolutionary relationships is known as
TLS Online TPP Program

#Question id: 14134

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

PAM stands for ___
TLS Online TPP Program

#Question id: 14135

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following statement is correct about PAM matrix? 
TLS Online TPP Program

#Question id: 14136

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following statement is incorrect about global alignment? 
(A) Global alignment is based on the Needleman–Wunsch algorithm.
(B) In Global alignment algorithm, an optimal alignment is obtained over the entire lengths of the two sequences.
(C) It is only suitable for aligning two closely related sequences that are of the same length.
(D) GAP is a web-based pairwise global alignment program.
TLS Online TPP Program

#Question id: 14137

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Consider the following alignment of two DNA sequences:
                            AGTAAC
                            AA_ _AC

Assuming an affine gap scoring scheme of an identity matrix for substitution, a gap initiation penalty of 1 and a gap extension penalty of 0.1, the score of the alignment is _________