Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 1280

#Section 3: Genetics, Cellular and Molecular Biology

Cellular responses to adhesion receptor signaling do not include:

#Question id: 3630

#Section 3: Genetics, Cellular and Molecular Biology

In the pearl-millet plant, color is determined by three alleles at a single locus: Rp1 (red), Rp2 (purple), and rp (green). Red is dominant over purple and green, and purple is dominant over green (Rp1 > Rp2 > rp). What will be expected phenotypes and ratios of offspring produced by the Rp1/rp × Rp2/rp crosses?

#Question id: 21163

#Section 5: Bioprocess Engineering and Process Biotechnology

#Question id: 1128

#Section 3: Genetics, Cellular and Molecular Biology

During the epinephrine stimulated conversion of glycogen to glucose 1-phosphate, which of the following steps involves protein phosphorylation?

a. inactivation of glycogen synthase               b. activation of protein kinase A

c. activation of adenylyl cyclise                      d. activation of glycogen phosphorylase

#Question id: 1867

#Section 2: General Biology

Which of the following is a coreceptor for HIV?