Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 14182

#Section 5: Bioprocess Engineering and Process Biotechnology

Aerobic degradation of benzoic acid by a mixed culture of microorganisms can be represented by the following reaction.

                Determine the yield coefficients YX/O2

#Question id: 14183

#Section 5: Bioprocess Engineering and Process Biotechnology

Aerobic degradation of benzoic acid by a mixed culture of microorganisms can be represented by the following reaction.

                Determine degree of reduction for the substrate.

   ______________

#Question id: 14184

#Section 5: Bioprocess Engineering and Process Biotechnology

Aerobic degradation of benzoic acid by a mixed culture of microorganisms can be represented by the following reaction.

             Determine degree of reduction for bacteria.

______________________

#Question id: 14185

#Section 5: Bioprocess Engineering and Process Biotechnology

Aerobic degradation of an organic compound by a mixed culture of organisms in waste water can be represented by the following reaction

                Determine a, b, c, d, and e, if YX/S = 0.4 g X/g S.

___________________

#Question id: 14186

#Section 5: Bioprocess Engineering and Process Biotechnology

Aerobic degradation of an organic compound by a mixed culture of organisms in waste water can be represented by the following reaction

                Determine the yield coefficients YX/O2

           ________________

#Question id: 14187

#Section 5: Bioprocess Engineering and Process Biotechnology

Aerobic degradation of an organic compound by a mixed culture of organisms in waste water can be represented by the following reaction

                Determine the yield coefficient YX/NH3.