Precision will be reduced, but yield will be increased Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following is a codominant marker?

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#Question id: 3897

#Section 3: Genetics, Cellular and Molecular Biology

Gene silencing by RNA interference acts by of the target gene.

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#Question id: 27910

#Section 5: Bioprocess Engineering and Process Biotechnology

The rate of oxygen tranfer is given by equation 

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#Question id: 1136

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following signaling pathways can be activated by activation of receptor tyrosine kinases?

a. phospholipase Cγ                b. PI-3 kinase

c. MAP kinase                         d. none of the above

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#Question id: 15254

#Section 6: Plant, Animal and Microbial Biotechnology

For growth, most bacteria require water activity levels above

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#Question id: 14132

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The most widely used program for multiple sequence alignment is