Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following techniques doesn’t involve use of a secondary antibody?

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#Question id: 18691

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The attractive interactions lead to ______energy.

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#Question id: 2092

#Section 3: Genetics, Cellular and Molecular Biology

Peripheral membrane proteins

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#Question id: 15459

#Section 6: Plant, Animal and Microbial Biotechnology

In the growth curve of a bacteria population, the bacteria are rapidly increasing in number in the

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#Question id: 224

#Section 2: General Biology

Which type of atom is involved in the reactive center of lipoamide?

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#Question id: 14974

#Section 1: Engineering Mathematics

Laplace transform of t² sin (2t).