Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

#Question id: 3697

#Section 3: Genetics, Cellular and Molecular Biology

In the Meselson-Stahl DNA replication experiment, what percent of the DNA was composed of one light strand and one heavy strand after one generation of growth in 14N containing growth media?

#Question id: 3698

#Section 3: Genetics, Cellular and Molecular Biology

In this diagram of the process of DNA replication at a replication fork, the strand labeled B is the:

#Question id: 3699

#Section 3: Genetics, Cellular and Molecular Biology

In this diagram of the process of DNA replication at a replication fork, the black boxes labeled D and E are:

#Question id: 3700

#Section 3: Genetics, Cellular and Molecular Biology

In the Meselson-Stahl DNA replication experiment, if the cells were first grown for many generations in 15N containing media, and then switched to 14N containing media, what percent of the DNA had 1 light strand and 1 heavy strand after 2 generations of growth in 15N growth media?

#Question id: 3701

#Section 3: Genetics, Cellular and Molecular Biology

Frederick Griffith accidentally discovered transformation when attempting to develop a vaccine for pneumonia. He injected mice with samples from S-strain (virulent) and/or R-strain (nonvirulent) pneumococci bacteria (Streptococcus pneumoniae). Which of the following results is NOT consistent with Griffith's experiments?

#Question id: 3702

#Section 3: Genetics, Cellular and Molecular Biology

For the DNA strand 5'-TACGATCATAT-3' the correct complementary DNA strand is: