Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

#Question id: 4741

#Section 3: Genetics, Cellular and Molecular Biology

The F2 progeny from a particular cross exhibit a modified dihybrid ratio of 9 : 7 (instead of 9 : 3 : 3 : 1). What phenotypic ratio would be expected from a testcross of the F1?

#Question id: 4745

#Section 3: Genetics, Cellular and Molecular Biology

In some goats, the presence of horns is produced by an autosomal gene that is dominant in males and recessive in females. A horned female is crossed with a hornless male. The F1 offspring are intercrossed to produce the F2. What proportion of the F2 offspring will have horns?

#Question id: 4746

#Section 3: Genetics, Cellular and Molecular Biology

Two independent pure line varieties having deep red and brown phenotype in their seed coat were cross, the all f1 progeny is deep red. The F2 progeny obtain from selfing show deep red and brown in ratio of 15:1. Which of the following observation is correct?

#Question id: 4747

#Section 3: Genetics, Cellular and Molecular Biology

How does incomplete dominance differ from incomplete penetrance?

#Question id: 4748

#Section 3: Genetics, Cellular and Molecular Biology

Eye color in Drosophila present on X chromosome, Red eye color is dominant over white eye color. There is non disjunction in Meiosis I in female and normal meiosis in male. Female is white eye color was cross with red eye male. What will be expected phenotype in their progeny?

#Question id: 4749

#Section 3: Genetics, Cellular and Molecular Biology

A normal woman, whose father was Hemophilic and color blind is married to a colorblind, nonhemophilic man. What will be probability of colorblind, hemophilic daughter?