Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

#Question id: 2002

#Section 2: General Biology

What accounts for antibody switching (i.e., the switch of one B cell from producing one class of antibody to another antibody class that is responsive to the same antigen)?

#Question id: 14400

#Section 5: Bioprocess Engineering and Process Biotechnology

internal mass transfer effects are eliminated when the internal effective ness factor is 

#Question id: 10540

#Section 6: Plant, Animal and Microbial Biotechnology

The following statements are made regarding volatile compounds of plant;

a) secondary metabolites also fuction in defence mechanism but this is not the volatile compound

b) These volatiles can act as cues for natural enemies of the insect herbivore such as parasitic wasps

c) All plants releases green-leaf volatiles in response to mechanical damage that is a mixture of aldehydes, alcohols, and esters

d) These volatiles attracts natural enemies of the attacking insect herbivores—predators or parasites

Which one of the following options represents a combination of correct statements?

#Question id: 149

#Section 2: General Biology

The pKaʹs of the side chain group and the α-carboxyl group of glutamate are 4.1 and 2.1, respectively. Which statement accounts for this difference?

#Question id: 565

#Section 2: General Biology

A transition-state analog: