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TLS Online TPP Program

#Question id: 13061

Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Arrange in the order of their increasing capacity:

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. Plasmid, BAC, Replacement vectors, Insertion vectors.
  2. Replacement, BAC, Insertion, Plasmid
  3. BAC, Insertion, Replacement, Plasmid
  4. Plasmid, Insertion, Replacement, BAC
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TLS Online TPP Program

#Question id: 27186

#Section 5: Bioprocess Engineering and Process Biotechnology

If the zero order reaction takes 100 min for 50% completion, how much time will it take for the 75% completion of the reaction?
TLS Online TPP Program

#Question id: 27187

#Section 5: Bioprocess Engineering and Process Biotechnology

A zero order reaction in A has a rate constant of 0.003 molL-1sec-1. How long will it take for A’s initial concentration to fall from 0.10 M to 0.075 M?
TLS Online TPP Program

#Question id: 27188

#Section 5: Bioprocess Engineering and Process Biotechnology

k for a zero order reaction is 2×10-2 mol/L s. If the concentration of the reactant after 25s is 0.5 M. Calculate the initial concentration of the reactant.
TLS Online TPP Program

#Question id: 27189

#Section 5: Bioprocess Engineering and Process Biotechnology

How long will it take for the initial concentration of A to fall from 0.20 M to 0.075, if the rate constant for a reaction of zero-order in A is 0.035 mol L–1 s–1.
TLS Online TPP Program

#Question id: 27190

#Section 5: Bioprocess Engineering and Process Biotechnology

A substrate is consumed in a zero order reaction such that the concentration falls from 40 g/l to 20 g/l in 4 h. How long will it take the substrate to fall from 20 g/l to 2 g/l:
TLS Online TPP Program

#Question id: 27191

#Section 5: Bioprocess Engineering and Process Biotechnology

The half-life of a zero order reaction is 30 minutes. What is the concentration of the reactant left after 60 minutes?