Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 12819

#Section 2: General Biology

The length and breadth of a rectangle are increased by 20% and 25% respectively. The increase in the area of the resulting rectangle will be :

TLS Online TPP Program

#Question id: 12902

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

An extension of microarray technology may also be used to analyze tissue sections,this process is known as_____

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#Question id: 12903

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

A gene isolation system that uses adapted vectors termed as_______

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#Question id: 12904

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:

1. Southern Blotting	i. Melting temperature of DNA strand
2. DNA microchips	ii. oligonucleotide matching to DNA sample
3. ASO-PCR	iii. DNA hybridization to oligo arrays
4. DGGE	iv. labeled probe hybridization to DNA

TLS Online TPP Program

#Question id: 12905

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Some gene/protein is used to identify the Disorder in humans Match them correctly:

1. Tyrosine Kinase(TK)	i. Atherosclerosis
2. HIV transactivator	ii. Hypertension
3. Angiotensinogen	iii. Kaposis Sarcoma
4. CET protein	iv. Cardiac Hypertrophy

TLS Online TPP Program

#Question id: 12906

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

An alternative method labeling strategy used in karyotyping and gene localisation is _______