Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 4831

#Section 3: Genetics, Cellular and Molecular Biology

There are 4 pairs of chromosomes in a Drosophila.The linkage groups present in it are

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#Question id: 14554

#Section 1: Engineering Mathematics

The series 

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#Question id: 14321

#Section 5: Bioprocess Engineering and Process Biotechnology

Both Saccharomyces cerevisiae yeast and Zymomonas mobilis bacteria produce ethanol from glucose under anaerobic conditions without external electron acceptors. The biomass yield from glucose is 0.11 g g-1 for yeast and 0.05 g g-I for Z. mobilis. In both cases the nitrogen source is NH 3. Both cell compositions are represented by the formula CH 1.800.5N0.2. Compare actual yield with theoretical maximum for Zymomonas mobilis bacteria ?

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#Question id: 10264

#Section 3: Genetics, Cellular and Molecular Biology

Chiasmata formation occurs during

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#Question id: 15518

#Section 1: Engineering Mathematics

The general solution of the differential equation in terms of arbitrary  constants K1 and K2 is: