Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

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#Question id: 8930

#Section 6: Plant, Animal and Microbial Biotechnology

Soon after the coelom begins to form, a researcher injects a dye into the coelom of a deuterostome embryo. Initially, the dye should be able to flow directly into the

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#Question id: 27228

#Section 3: Genetics, Cellular and Molecular Biology

The structure of RNA differs from that of DNA, as RNA contains:

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#Question id: 15512

#Section 1: Engineering Mathematics

Solution of y" + 6y' + 9y is 

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#Question id: 16016

#Section 6: Plant, Animal and Microbial Biotechnology

Range of optimum glutamine concentration present in the culture media is

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#Question id: 16786

#Section 1: Engineering Mathematics

What is the median of 7.1, 3.2, 5.8, 8.9, 6.5, 10.2, 9, 12.5