Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

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#Question id: 27472

#Section 5: Bioprocess Engineering and Process Biotechnology

The restriction of enzyme mobility in a fixed space is known as

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#Question id: 1126

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following statement(s) is (are) true of the α subunit of a G protein?

a. It physically associates with the receptor.              

b. It binds GDP.

c. It binds GTP.                     

d. It is a transmembrane protein.

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#Question id: 14473

#Section 1: Engineering Mathematics

The absolute minimum of   on the interval [ -1, 1] is at

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#Question id: 3927

#Section 3: Genetics, Cellular and Molecular Biology

An operon is __

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#Question id: 2785

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following terms describes when a chromosome is replicated everywhere except the telomeres and centromere, but the daughter chromosomes do not separate?