Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

#Question id: 28114

#Section 5: Bioprocess Engineering and Process Biotechnology

Which is the most frequently used impeller in the fermentation industry regarded as 6- flat-blade disc-mounted turbine?

#Question id: 28115

#Section 5: Bioprocess Engineering and Process Biotechnology

Transient flow range of mixing speed is obtained for

#Question id: 28116

#Section 5: Bioprocess Engineering and Process Biotechnology

A baffled fermenter with tank diameter and liquid height equal to 1.2 m is stirred using a six-blade downward-pumping impeller. The impeller diameter is 0.42 m and the stirrer speed is 1.5 s. The viscosity of the fermentation broth is 10^-2 Pa s and the density is 1000 kg m-³. Estimate the mixing time under nonaerated conditions.

#Question id: 28150

#Section 5: Bioprocess Engineering and Process Biotechnology

A purified hydrolytic enzyme (Mr 150 000) is available with the description: Gives a Vmax of 3 200 units/mg protein under optimal conditions. How many micromoles of substrate can be hydrolysed by 1 mg of protein in 1 minute  

#Question id: 31198

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 31199

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology