Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

#Question id: 11801

#Section 6: Plant, Animal and Microbial Biotechnology

Which one of the following compound most commonly transported in sieve elements of the phloem?

#Question id: 11802

#Section 6: Plant, Animal and Microbial Biotechnology

Damaged sieve elements are sealed off, but because of the processs that plug sieve plates pores, excuded phloem sap from wounds that sever sieve elements. Exudation of sap enhanced by the inclusion of;

#Question id: 11803

#Section 6: Plant, Animal and Microbial Biotechnology

Due to damage exudation of sap from cut petioles or stems takes place they can be collected. However, quantities of collected sap are small, and the method is technically difficult. A preferable approach for collecting exuded sap is to use an

#Question id: 11804

#Section 6: Plant, Animal and Microbial Biotechnology

P-proteins have been characterized at the molecular level. P-proteins from the genus Cucurbita consist of two major proteins;

#Question id: 11805

#Section 6: Plant, Animal and Microbial Biotechnology

P-proteins from the genus Cucurbita consist of two major proteins: PP1, the phloem filament protein, and PP2, the phloem lectin. Both PP1 and PP2 are thought to be synthesized in_______A___________and transported via the_______B_________to the_______C______, where they associate to form P-protein filaments and P-protein bodies.

#Question id: 11806

#Section 6: Plant, Animal and Microbial Biotechnology

sap is translocated from areas of supply, called sources, to areas of metabolism or storage, called sinks. Which of the following statements about source and sink are TRUE?

a.) Sinks include any nonphotosynthetic organs of the plant and organs that do not produce enough photosynthetic products to support their own growth or storage needs

b.) Sources include exporting organs that are capable of producing photosynthate in excess of their own needs

c.) During the growing season of the first year, storage root is a sink, when it accumulates sugars received from the source leaves. During the second growing season the same root becomes a source

d.) Roots, tubers, developing fruits, and immature leaves, which must import carbohydrate for normal development, are all examples of sources tissues

Find the correct combination of the source and sink pattern of translocation.