Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

TLS Online TPP Program

#Question id: 2095

#Section 3: Genetics, Cellular and Molecular Biology

Glycolipids and glycoproteins are especially abundant in the

TLS Online TPP Program

#Question id: 2095

#Section 2: General Biology

Glycolipids and glycoproteins are especially abundant in the

TLS Online TPP Program

#Question id: 2096

#Section 3: Genetics, Cellular and Molecular Biology

Movement of phospholipids from one leaflet to the other

TLS Online TPP Program

#Question id: 2096

#Section 2: General Biology

Movement of phospholipids from one leaflet to the other

TLS Online TPP Program

#Question id: 2097

#Section 3: Genetics, Cellular and Molecular Biology

Free and unesterified fatty acids bind to one of a number of chaperone proteins in order to be transported in the aqueous environment of the cytosol. Which of the following facilitates this intracellular transport?

TLS Online TPP Program

#Question id: 2098

#Section 3: Genetics, Cellular and Molecular Biology

The major site of lipid synthesis in eukaryotic cells is the