Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 14498

#Section 1: Engineering Mathematics

Match the following :

   

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#Question id: 13041

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following is incorrect about Data Collection? 

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#Question id: 3885

#Section 3: Genetics, Cellular and Molecular Biology

AZT (3'-azido-2',3'-dideoxythymidine), used to treat HIV infection, acts in HIV-infected cells by:

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#Question id: 151

#Section 2: General Biology

The isoelectric point of alanine is pH = 6.15. It is mixed with proline (pHCOOH = 2.0; pHNH2= 10.6), and the mixture is placed in an electric field at pH 6.15. Which statement is true?

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#Question id: 27229

#Section 3: Genetics, Cellular and Molecular Biology

The bond between the bases and the sugars in DNA referred as: