According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

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#Question id: 16012

#Section 6: Plant, Animal and Microbial Biotechnology

Which of the followings are the metabolic products of glucose and glutamine?

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#Question id: 3953

#Section 3: Genetics, Cellular and Molecular Biology

Which statement best characterizes the nuclear localization signal?

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#Question id: 14630

#Section 1: Engineering Mathematics

The series whose n^th term is sin 1/n is

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#Question id: 402

#Section 4: Fundamentals of Biological Engineering

If the DG'° of the reaction A -> B is –40 kJ/mol, under standard conditions the reaction:

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#Question id: 4817

#Section 3: Genetics, Cellular and Molecular Biology

How many different gametes can be formed by an organism with genotype AaBbCCddEe, If a, b and c gene are completely link?