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TLS Online TPP Program
#Question id: 17201
#Section 1: Engineering Mathematics
Company A produces 10% defective products, Company B produces 20% defective products and C produces 5% defective products. If choosing a company is an equally likely event, then find the probability that the product chosen is defective...........
TLS Online TPP Program
#Question id: 4132
#Section 3: Genetics, Cellular and Molecular Biology
In E. coli, aminoacyl-tRNA synthetases:
TLS Online TPP Program
#Question id: 13101
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
You are a scientist who is using genomics to currently study a new bacterial species that no one has ever studied before. The following sequence is a piece of DNA within the coding region of a gene that you have recently sequenced.
You are using shotgun sequencing to determine the DNA sequence of the genome of this new bacterial species. For one strand of a 30-nucleotide long stretch of DNA, you get the following sequences out of your shotgun sequencing reaction. Assemble the entire 30-nt-long DNA sequence
5’-TGGGAGTTCCTCAAACGCGTTGTCACTGAC-3’
You put the DNA sequence that you have assembled into a computer program that tells you that the following piece of DNA, which comes from another bacterium, is a close match to the sequence you have sequenced from your bacterium: 5’-…TGGGCATTTCTCAAGCGGGTTGTAATGGAT…-3’
This 30-nt-long sequence fragment lies in the center of a gene, and that portion of the sequence encodes for this 10-amino acid-long part of a protein:
N-…Trp-Ala-Phe-Leu-Lys-Arg-Val-Val-Met-Asp…-C
You hypothesize that the sequence you have discovered is another bacterial species’ version of the same gene as this previously known gene. To measure how identical the two genes are at the DNA level and/or the two proteins are at the amino acid level, you can calculate a percentage of “identity” for each. This is the percent of nucleotides (for the gene) or the percent of amino acids (for the protein) that are identical between the two sequences.
What is the % identity between the two protein sequences?
TLS Online TPP Program
#Question id: 15580
#Section 4: Fundamentals of Biological Engineering
The unit of volumetric oxygen transfer coefficient (KLa) is
TLS Online TPP Program
#Question id: 399
#Section 4: Fundamentals of Biological Engineering
Calculate the pH of a 1 L solution containing 0.100 M formic acid and 0.100 M sodium formate before and after the addition of 1.00 mL of 5.00 M NaOH. How much would the pH change if the NaOH were added to 1.00 L of pure water? (pKa for formic acid is 3.75)