#Question id: 14294
#Section 5: Bioprocess Engineering and Process Biotechnology
#Question id: 14295
#Section 5: Bioprocess Engineering and Process Biotechnology
Pseudomonas methylotrophus is used to produce single-cell
protein from methanol in a 1000 m3 pressure-cycle airlift fermenter.
The biomass yield from substrate is 0.41 g g- 1, KS is
0.7 mg l- 1, and the maximum specific growth rate is 0.44 h- 1.
The medium contains 4% (w/v) methanol. A substrate conversion of 98% is
desirable. The reactor may be operated either in batch or continuous mode. If
operated in batch, an inoculum of 0.01% (w/v) is used and the downtime between
batches is 20 h. If operated continuously, a downtime of 25 d is expected per
year. Neglecting maintenance requirements, compare the annual biomass
production from batch and continuous reactors?
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#Question id: 14296
#Section 5: Bioprocess Engineering and Process Biotechnology
A two-stage chemostat system is used for production of
secondary metabolite. The volume of each reactor is 0.5 m3; the flow
rate of feed is 50 l h- 1. Mycelial growth occurs in the first
reactor; the second reactor is used for product synthesis. The concentration of
substrate in the feed is 10 gl-1. Kinetic and yield parameters for
the organism are:
Assume that product synthesis is negligible in the first
reactor and growth is negligible in the second reactor. Determine cell
concentrations entering the second reactor.
#Question id: 14297
#Section 5: Bioprocess Engineering and Process Biotechnology
A two-stage chemostat system is used for production of
secondary metabolite. The volume of each reactor is 0.5 m3; the flow
rate of feed is 50 l h- 1. Mycelial growth occurs in the first
reactor; the second reactor is used for product synthesis. The concentration of
substrate in the feed is 10 gl-1. Kinetic and yield parameters for
the organism are:
Assume that product synthesis is negligible in the first
reactor and growth is negligible in the second reactor. Determine substrate
concentrations entering the second reactor.
#Question id: 14298
#Section 5: Bioprocess Engineering and Process Biotechnology
A two-stage chemostat system is used for production of
secondary metabolite. The volume of each reactor is 0.5 m3; the flow
rate of feed is 50 l h- 1. Mycelial growth occurs in the first
reactor; the second reactor is used for product synthesis. The concentration of
substrate in the feed is 10 gl-1. Kinetic and yield parameters for
the organism are:
Assume that product synthesis is negligible in the first
reactor and growth is negligible in the second reactor. What is the overall
substrate conversion?
#Question id: 14299
#Section 5: Bioprocess Engineering and Process Biotechnology
Assume that product synthesis is negligible in the first
reactor and growth is negligible in the second reactor. What is the final
concentration of product?