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TLS Online TPP Program

#Question id: 398

One of the buffers that contribute to pH stability in human blood is carbonic acid (H2CO3). Carbonic acid is a weak acid that, when placed in an aqueous solution, dissociates into a bicarbonate ion (HCO3-) and a hydrogen ion (H+). (See figure.)

If the pH of blood drops, one would expect ________.

#Section 4: Fundamentals of Biological Engineering

  1. a decrease in the concentration of H2CO3 and an increase in the concentration of HCO3-

  2. the concentration of bicarbonate ions (HCO3-) to increase

  3. the HCO3- to act as a base and remove excess H+ by the formation of H2CO3

  4. the HCO3- to act as an acid and remove excess H+ by the formation of H2CO3

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TLS Online TPP Program

#Question id: 12907

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The binding of a regulatory protein or transcription factor to a specific DNA site results in a complex which may be analyzed by the technique termed as _____
TLS Online TPP Program

#Question id: 12908

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which statement is incorrect about CRISPR?
TLS Online TPP Program

#Question id: 12909

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

According to the diagram, arrange the steps in correct order: 


1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 
2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 
3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).
 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.
TLS Online TPP Program

#Question id: 12910

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:

A. RNA analysisi. Sexing or cell mutation sitesa. Expressed Sequence tag
B. Forensic scienceii. mRNA analysisb. Analysis of DNA from blood
C. Gene discoveryiii. DNA profilingc. Active latent viral infections
D. Single-cell analysisiv. RT–PCRd. Sex determination of unborn
TLS Online TPP Program

#Question id: 12911

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:

A. DNA polymerasesi. Alkaline phosphatasea. RNA-dependent DNA polymerase, used in cDNA synthesis           
B. Reverse transcriptaseii. T4 DNA ligaseb. Thermostable DNA polymerase used in PCR
C. Ligasesiii. AMV-RTc. Removes 5'-phosphates from DNA and RNA
D. Phosphatasesiv. Taq DNA pold. Links 5'-phosphate and 3'-hydroxyl ends via phosphodiester bond
TLS Online TPP Program

#Question id: 12912

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Name the enzyme which separates the double-helical DNA into single strands.