Mycotoxins are formed during the end of

TLS Online TPP Program

#Question id: 27909

#Section 5: Bioprocess Engineering and Process Biotechnology

A 20-1 stirred fermenter containing a Bacillus thuringiensis culture at 30°C is used for production of microbial insecticide, ka is determined using the dynamic method. Air flow is shut off for a few minutes and the dissolved-oxygen level drops; the air supply is then re-connected. When steady state is established, the dissolved-oxygen tension is 78% air saturation. The following results are obtained. Estimate ka.

TLS Online TPP Program

#Question id: 13092

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

Which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously?

TLS Online TPP Program

#Question id: 12926

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

PCR is undertaken with a fluorescence reporter / quencher pair called______

TLS Online TPP Program

#Question id: 15256

#Section 6: Plant, Animal and Microbial Biotechnology

A low molecular weight molecules that are able to complex with ferric iron and supply it to the cell

TLS Online TPP Program

#Question id: 28108

#Section 5: Bioprocess Engineering and Process Biotechnology

Consider the following reversible reaction. In a 3.00 liter container, the following  amounts are found in equilibrium at 400 oC: 0.0420 mole N₂, 0.516 mole H₂ and 0.0357 mole NH3. Evaluate K_eq.

N2(g) + 3H2(g) ↔ 2NH3(g)