#Question id: 14267
#Section 5: Bioprocess Engineering and Process Biotechnology
In a trickling biological filter, the BOD value of the feed
stream is S0i = 500 mg/l with a feed flow of F = 103 l/h.
The effluent BOD value is desired to be S0 = 10 mg/l. The following
kinetic parameters for the biocatalysts are known: rm = 20 mg/S/l .h
and Ks = 200 mg S/l. The biofilm thickness is L = 0.1 mm. The
cross-sectional area of the filter is A = 2 m2 , and the biofilm
surface area per unit volume of the bed is a = 500 cm2 /cm3
. Assume that dissolved oxygen is the rate-limiting substrate and the diffusion
coefficient of oxygen is DO2 = 2 × 10-5 cm2
/s. Determine the required height of the bed. You can assume first-order
bioreaction kinetics?
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#Question id: 14268
#Section 5: Bioprocess Engineering and Process Biotechnology
An activated-sludge waste treatment system is required to reduce the amount of BOD5 from 1000 mg/l to 20 mg/l at the exit. The sedimentation unit concentrates biomass by a factor of 3. Kinetic parameters are µm = 0.2 h-1 , Ks = 80 mg/l, kd = 0.01 h-1 , and Y M X/S = 0.5 g MLVSS/g BOD5. The flow of waste water is 10000 l/h and the size of the treatment basin is 50,000 l. What is the value of the solids residence time?
#Question id: 14269
#Section 5: Bioprocess Engineering and Process Biotechnology
An activated-sludge waste treatment system is required to
reduce the amount of BOD5 from 1000 mg/l to 20 mg/l at the exit. The
sedimentation unit concentrates biomass by a factor of 3. Kinetic parameters
are µm
= 0.2 h-1 , Ks = 80 mg/l, kd = 0.01 h-1 , and Y M X/S
= 0.5 g MLVSS/g BOD5. The flow of waste water is 10000 l/h and
the size of the treatme nt basin is 50,000 l. What value of the recycle ratio
must be used?
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#Question id: 14270
#Section 5: Bioprocess Engineering and Process Biotechnology
Consider a well-mixed waste treatment system for a small-scale system. The system is operated with a reactor of 1000 l and flow rate of 100 l/h. The separator concentrates biomass by a factor of 2. The recycle ratio is 0.7. The kinetic parameters are µm = 0.5 h-1 , Ks = 0.2 g/l, Y M X/S = 0.5 g/g, and kd = 0.05 h-1 . What is the exit substrate concentration?
#Question id: 14271
#Section 5: Bioprocess Engineering and Process Biotechnology
Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. Determine the concentration of cells at quasi-steady state when t = 10 h.
#Question id: 14272
#Section 5: Bioprocess Engineering and Process Biotechnology
Penicillin is produced by P. chrysogenum in a fed-batch culture with the intermittent addition of glucose solution to the culture medium. The initial culture volume at quasi-steady state is V0 = 500 l, and glucose-containing nutrient solution is added with a flow rate of F = 50 l/h. Glucose concentration in the feed solution and initial cell concentration are S0 = 300 g/l and X0 = 20 g/l, respectively. The kinetic and yield coefficients of the organism are mm = 0.2 h-1 , KS = 0.5 g/l, and YX/S = 0.3 g dw/g glucose. If qP = 0.05 g product/g cells h and P0 = 0.1 g/l, determine the product concentration in the vessel at t = 10 h