Practice Questions

TLS Online TPP Program

#Question id: 14689

A determinant is chosen at random from the set of all determinants of order 2 with elements 0 or 1 only. The probability that value of the determinant chosen is
positive is

#Section 1: Engineering Mathematics

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1 / 17
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TLS Online TPP Program

#Question id: 3013

#Section 2: General Biology

Bacteria survive adverse conditions by forming

TLS Online TPP Program

#Question id: 10127

#Section 2: General Biology

In higher plants, the multi-subunit protein supercomplex has two complete reaction centers and some antenna complexes. The core of the reaction center consists of two membrane proteins known as D1 and D2, Which contained a multi-subunit protein supercomplexes;

TLS Online TPP Program

#Question id: 15890

#Section 6: Plant, Animal and Microbial Biotechnology

Tissue culture was developed by _______ but the concept of tissue culture was conceived by ______

TLS Online TPP Program

#Question id: 13088

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.