Gel chromatography is used for hormone separation. A pilot-plant scale gel chromatography column packed with sephacryl resin is used to separate two hormones, A and B. The column is 5 cm in diameter and 30 cm in height: V, = 1.9x 10-⁴m³, Wr = 3x10^-3m³ /kg dry sephacryl, ps =1.25 x 10³ kg/m³ . The partition coefficients are k_pA =0.38 and k_pB= 0.15. If the eluting flow rate is 0.7 I/h, what is the retention time for each hormone? 

#Question id: 14174

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of baker’s yeast (S. cerevisiae) on glucose may be simply described by the following equation:

In a batch reactor of volume 10^5 l, the final desired yeast concentration is 50 gdw/l. Using the above reaction stoichiometry, Determine the total amount of oxygen required?

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#Question id: 14175

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction

Determine the biomass yield coefficient YX/S? 

#Question id: 14176

#Section 5: Bioprocess Engineering and Process Biotechnology

 The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction

Determine the product yield coefficient YEtOH/S 

#Question id: 14177

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction

Determine the product yield coefficients YCO2/S 

#Question id: 14178

#Section 5: Bioprocess Engineering and Process Biotechnology

#Question id: 14179

#Section 5: Bioprocess Engineering and Process Biotechnology

The growth of S. cerevisiae on glucose under anaerobic conditions can be described by the following overall reaction: 

Determine the coefficient R.