Gel chromatography is used for hormone separation. A pilot-plant scale gel chromatography column packed with sephacryl resin is used to separate two hormones, A and B. The column is 5 cm in diameter and 30 cm in height: V, = 1.9x 10-⁴m³, Wr = 3x10^-3m³ /kg dry sephacryl, ps =1.25 x 10³ kg/m³ . The partition coefficients are k_pA =0.38 and k_pB= 0.15. If the eluting flow rate is 0.7 I/h, what is the retention time for each hormone? 

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#Question id: 14402

#Section 5: Bioprocess Engineering and Process Biotechnology

Baby hamster kidney cells are immobilised in alginate beads. The average particle diameter is 5 mm. Rate of oxygen consumption at a bulk concentration of 8 x 10 ^-3 kg 0 2 m ^-3 is 8.4 x 10 ^-5 kg s^- 1 m^-3 catalyst. The effective diffusivity of oxygen in the beads is 1.88 x 10^ -9 m 2 s^- 1. Assume that the oxygen concentration at the surface of the catalyst is equal to the bulk concentration, and that oxygen uptake follows zero-order kinetics. calculate the observable Thiele modulus. 

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#Question id: 17817

#Section 4: Fundamentals of Biological Engineering

Power number is expressed as

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#Question id: 3589

#Section 3: Genetics, Cellular and Molecular Biology

Monohybrid Plant having genotype Tt were allowed to test cross , what is probability of random three plant selected that is

A- two tall, one dwarf      B- one tall, two dwarf       C- all tall

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#Question id: 5267

#Section 3: Genetics, Cellular and Molecular Biology

The F plasmid carries the information for

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#Question id: 2956

#Section 3: Genetics, Cellular and Molecular Biology

The product of the cdc2 gene is