#Question id: 14243
#Section 5: Bioprocess Engineering and Process Biotechnology
A tubular-bowl centrifuge is used to concentrate a suspension of genetically-engineered yeast containing a new recombinant protein. At a speed of 12 000 rpm, the centrifuge treats 3 l broth min-1 with satisfactory results. It is proposed to use the same centrifuge to separate cell debris from homogenate produced by mechanical disruption of the yeast. If the average size of the debris is one-third that of the yeast and the viscosity of the homogenate is five times greater than the cell suspension, what flow rate can be handled if the centrifuge is operated at the same speed? _____________________
#Question id: 2065
#Section 3: Genetics, Cellular and Molecular Biology
#Question id: 21728
#Section 5: Bioprocess Engineering and Process Biotechnology
#Question id: 1598
#Section 2: General Biology
Which of the following polypeptide is important for the expression of MHC I on the cell membrane?
#Question id: 14273
#Section 5: Bioprocess Engineering and Process Biotechnology
Nicotiana tabacum cells are cultured to high density for
production of polysaccharide gum. The reactor used is a stirred tank,
containing initially 100 litres medium. The maximum specific growth rate of the
culture is 0.18 d-1 and the yield of biomass from substrate is 0.5 g
g-l. The concentration of growth-limiting substrate in the medium is
3% (w/v). The reactor is inoculated with 1.5 g l- 1 cells and
operated in batch until the substrate is virtually exhausted; medium flow is
then started at a rate of 4 l d-1. Fed-batch operation occurs under
quasi-steady-state conditions. Estimate the batch culture time. ____________________