Practice Questions

TLS Online TPP Program

#Question id: 14861

A fermentation slurry containing Streptomyces kanamyceticus cells is filtered using a continuous rotary vacuum filter. 120 kg h^{- 1} slurry is fed to the filter; 1 kg slurry contains 60 g cell solids. To improve filtration rates, particles of diatomaceous-earth filter aid are added at a rate of 10 kg h^{- 1}. The concentration of kanamycin in the slurry is 0.05% by weight. Liquid filtrate is collected at a rate of 112 kg h^-1; the concentration of kanamycin in the filtrate is 0.045% (w/w). Filter cake containing cells and filter aid is continuously removed from the filter cloth.
If the concentration of kanamycin in the filter-cake liquid is the same as in the filtrate, how much kanamycin is absorbed per kg filter aid?.,..... 10^-4

#Section 4: Fundamentals of Biological Engineering

More Questions

#Question id: 14335

#Section 5: Bioprocess Engineering and Process Biotechnology

A cylindrical stirred bioreactor of diameter and height 2 m has a Rushton turbine one-third the tank diameter in size. The bioreactor contains Newtonian culture broth with the same density as water and with viscosity 4 cP. If the specific power consumption must not exceed 1.5 kW m^-3, determine the maximum allowable stirrer speed. What is the mixing time under these conditions? __________

TLS Online TPP Program

#Question id: 14336

#Section 5: Bioprocess Engineering and Process Biotechnology

A cylindrical stirred bioreactor of diameter and height 2 m has a Rushton turbine one-third the tank diameter in size. The bioreactor contains Newtonian culture broth with the same density as water and with viscosity 4 cP. The tank is now aerated. In the presence of gas bubbles, the approximate relationship between ungassed power number (N_P)₀ and gassed power number (N_P)g is: (N_P)g =0.5 (N_P)₀. What maximum stirrer speed is now possible in the sparged reactor? Estimate the mixing time.

TLS Online TPP Program

#Question id: 14337

#Section 5: Bioprocess Engineering and Process Biotechnology

A fermentation broth with viscosity 10 −2 Pa s and density 1000 kg m − 3 is agitated in a 2.7 m^ 3 baffled tank using a Rushton turbine with diameter 0.5 m and stirred speed 1 s ^−1. Estimate the mixing time.

TLS Online TPP Program

#Question id: 14338

#Section 5: Bioprocess Engineering and Process Biotechnology

Quasi steady state condition is

TLS Online TPP Program

#Question id: 14339

#Section 5: Bioprocess Engineering and Process Biotechnology

In continuous culture which phase is usually longer?