TLS Online TPP Program

#Question id: 15869


In a modest laboratory, aseptic handling, maintenance and observation of cultures, these activities can be done in the culture room.  Some facilities are required for the culture room, except?

#Section 6: Plant, Animal and Microbial Biotechnology
  1. Controlled temperature
  2. Culture racks fitted with light
  3. Shaker for agitation of liquid cultures
  4. Controlled pH
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TLS Online TPP Program

#Question id: 651

#Section 2: General Biology

Consider an enzyme catalysed reaction treated with inhibitor. Enzyme kinetic was analysed with Eadie Hofstee equation. A plot of V0 versus V0/[S] for an enzyme-catalyzed reaction is shown below. The control curve was obtained in the absence of inhibitor. Which effect is shown on the enzyme activity when an inhibitor is added to the reaction mixture?

TLS Online TPP Program

#Question id: 5246

#Section 3: Genetics, Cellular and Molecular Biology

The end result of F factor mediated conjugation:

TLS Online TPP Program

#Question id: 4344

#Section 3: Genetics, Cellular and Molecular Biology

How does the transcription of structural genes in an inducible operon occur?

TLS Online TPP Program

#Question id: 14861

#Section 4: Fundamentals of Biological Engineering

A fermentation slurry containing Streptomyces kanamyceticus cells is filtered using a continuous rotary vacuum filter. 120 kg h- 1 slurry is fed to the filter; 1 kg slurry contains 60 g cell solids. To improve filtration rates, particles of diatomaceous-earth filter aid are added at a rate of 10 kg h- 1. The concentration of kanamycin in the slurry is 0.05% by weight. Liquid filtrate is collected at a rate of 112 kg h-1; the concentration of kanamycin in the filtrate is 0.045% (w/w). Filter cake containing cells and filter aid is continuously removed from the filter cloth.
If the concentration of kanamycin in the filter-cake liquid is the same as in the filtrate, how much kanamycin is absorbed per kg filter aid?.,..... 10-4

TLS Online TPP Program

#Question id: 14281

#Section 5: Bioprocess Engineering and Process Biotechnology

cells are grown in a CSTR a steady state at two dilution rates 0.1 h^-1 and 0.5h^-1 and the steady state concentrations are as follows: 


if the inlet substrate concentration is 20 g/I, the cells follow monod growth kinetics and product formation kinetics is modelled as a both growth associated and non growth associated kinetics given by the equation then answer the following questions, the best estimate of maximum specific growth rate is: