Small organic molecules that usually make up all or part of enzyme cofactors and only very small amounts sustain growth

TLS Online TPP Program

#Question id: 318

#Section 2: General Biology

Attractions of oppositely charged functional groups of proteins are sometimes called ________.

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#Question id: 27474

#Section 5: Bioprocess Engineering and Process Biotechnology

When NDa <<1, the overall reaction is controlled by

TLS Online TPP Program

#Question id: 3596

#Section 3: Genetics, Cellular and Molecular Biology

Which of the following correct regarding test cross?

a) A testcross tests reveals the genotype of the individual

b)  Test cross detect linkage between gene

c) Same genotype and phenotype ratio present in test cross progeny

d) Phenotype ratio of test cross used to determine mode of inheritance either complete dominance or incomplete dominance

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#Question id: 14212

#Section 5: Bioprocess Engineering and Process Biotechnology

Which fig represents Kinetic patterns of non growth associated product formation 

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#Question id: 13091

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?